Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_32psu_4_S8_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_32psu_4_S8_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-1 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 59451 Mapping efficiency: 59.5% Sequence pairs with no alignments under any condition: 23988 Sequence pairs did not map uniquely: 16561 Sequence pairs which were discarded because genomic sequence could not be extracted: 14 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 14726 ((converted) top strand) GA/CT/CT: 14877 (complementary to (converted) top strand) GA/CT/GA: 15105 (complementary to (converted) bottom strand) CT/GA/GA: 14729 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2814290 Total methylated C's in CpG context: 198435 Total methylated C's in CHG context: 10514 Total methylated C's in CHH context: 52328 Total methylated C's in Unknown context: 7823 Total unmethylated C's in CpG context: 74973 Total unmethylated C's in CHG context: 669381 Total unmethylated C's in CHH context: 1808659 Total unmethylated C's in Unknown context: 29095 C methylated in CpG context: 72.6% C methylated in CHG context: 1.5% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 21.2% Bismark completed in 0d 0h 1m 35s