Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_26psu_3_S11_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/8C_26psu_3_S11_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-1 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 61863 Mapping efficiency: 61.9% Sequence pairs with no alignments under any condition: 15515 Sequence pairs did not map uniquely: 22622 Sequence pairs which were discarded because genomic sequence could not be extracted: 14 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 15846 ((converted) top strand) GA/CT/CT: 14752 (complementary to (converted) top strand) GA/CT/GA: 14960 (complementary to (converted) bottom strand) CT/GA/GA: 16291 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2447940 Total methylated C's in CpG context: 195717 Total methylated C's in CHG context: 10515 Total methylated C's in CHH context: 63093 Total methylated C's in Unknown context: 6642 Total unmethylated C's in CpG context: 65002 Total unmethylated C's in CHG context: 621924 Total unmethylated C's in CHH context: 1491689 Total unmethylated C's in Unknown context: 22499 C methylated in CpG context: 75.1% C methylated in CHG context: 1.7% C methylated in CHH context: 4.1% C methylated in unknown context (CN or CHN): 22.8% Bismark completed in 0d 0h 1m 40s