Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/CTRL_8C_26psu_1_S17_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/CTRL_8C_26psu_1_S17_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 65049 Mapping efficiency: 65.0% Sequence pairs with no alignments under any condition: 16751 Sequence pairs did not map uniquely: 18200 Sequence pairs which were discarded because genomic sequence could not be extracted: 13 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 16747 ((converted) top strand) GA/CT/CT: 15782 (complementary to (converted) top strand) GA/CT/GA: 16127 (complementary to (converted) bottom strand) CT/GA/GA: 16380 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2858093 Total methylated C's in CpG context: 187448 Total methylated C's in CHG context: 10595 Total methylated C's in CHH context: 68031 Total methylated C's in Unknown context: 7842 Total unmethylated C's in CpG context: 67624 Total unmethylated C's in CHG context: 660869 Total unmethylated C's in CHH context: 1863526 Total unmethylated C's in Unknown context: 32816 C methylated in CpG context: 73.5% C methylated in CHG context: 1.6% C methylated in CHH context: 3.5% C methylated in unknown context (CN or CHN): 19.3% Bismark completed in 0d 0h 1m 47s