Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_2_S2_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_2_S2_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 66670 Mapping efficiency: 66.7% Sequence pairs with no alignments under any condition: 13370 Sequence pairs did not map uniquely: 19960 Sequence pairs which were discarded because genomic sequence could not be extracted: 13 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 17693 ((converted) top strand) GA/CT/CT: 15633 (complementary to (converted) top strand) GA/CT/GA: 15721 (complementary to (converted) bottom strand) CT/GA/GA: 17610 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2783055 Total methylated C's in CpG context: 194866 Total methylated C's in CHG context: 12755 Total methylated C's in CHH context: 101865 Total methylated C's in Unknown context: 9723 Total unmethylated C's in CpG context: 73101 Total unmethylated C's in CHG context: 673425 Total unmethylated C's in CHH context: 1727043 Total unmethylated C's in Unknown context: 33439 C methylated in CpG context: 72.7% C methylated in CHG context: 1.9% C methylated in CHH context: 5.6% C methylated in unknown context (CN or CHN): 22.5% Bismark completed in 0d 0h 1m 38s