Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/CTRL_16C_26psu_2_S21_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/CTRL_16C_26psu_2_S21_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 28259 Mapping efficiency: 28.3% Sequence pairs with no alignments under any condition: 59082 Sequence pairs did not map uniquely: 12659 Sequence pairs which were discarded because genomic sequence could not be extracted: 4 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7453 ((converted) top strand) GA/CT/CT: 6642 (complementary to (converted) top strand) GA/CT/GA: 6589 (complementary to (converted) bottom strand) CT/GA/GA: 7571 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1142409 Total methylated C's in CpG context: 102702 Total methylated C's in CHG context: 3078 Total methylated C's in CHH context: 10772 Total methylated C's in Unknown context: 386 Total unmethylated C's in CpG context: 27432 Total unmethylated C's in CHG context: 297037 Total unmethylated C's in CHH context: 701388 Total unmethylated C's in Unknown context: 773 C methylated in CpG context: 78.9% C methylated in CHG context: 1.0% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 33.3% Bismark completed in 0d 0h 1m 15s