Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_4_S4_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_4_S4_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 33128 Mapping efficiency: 33.1% Sequence pairs with no alignments under any condition: 56167 Sequence pairs did not map uniquely: 10705 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8261 ((converted) top strand) GA/CT/CT: 8361 (complementary to (converted) top strand) GA/CT/GA: 8202 (complementary to (converted) bottom strand) CT/GA/GA: 8304 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1561643 Total methylated C's in CpG context: 109018 Total methylated C's in CHG context: 2899 Total methylated C's in CHH context: 11319 Total methylated C's in Unknown context: 309 Total unmethylated C's in CpG context: 32817 Total unmethylated C's in CHG context: 377817 Total unmethylated C's in CHH context: 1027773 Total unmethylated C's in Unknown context: 935 C methylated in CpG context: 76.9% C methylated in CHG context: 0.8% C methylated in CHH context: 1.1% C methylated in unknown context (CN or CHN): 24.8% Bismark completed in 0d 0h 1m 21s