Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_3_S3_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_32psu_3_S3_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 35217 Mapping efficiency: 35.2% Sequence pairs with no alignments under any condition: 48948 Sequence pairs did not map uniquely: 15835 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9209 ((converted) top strand) GA/CT/CT: 8447 (complementary to (converted) top strand) GA/CT/GA: 8239 (complementary to (converted) bottom strand) CT/GA/GA: 9320 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1415959 Total methylated C's in CpG context: 110893 Total methylated C's in CHG context: 3080 Total methylated C's in CHH context: 14760 Total methylated C's in Unknown context: 320 Total unmethylated C's in CpG context: 31743 Total unmethylated C's in CHG context: 364140 Total unmethylated C's in CHH context: 891343 Total unmethylated C's in Unknown context: 713 C methylated in CpG context: 77.7% C methylated in CHG context: 0.8% C methylated in CHH context: 1.6% C methylated in unknown context (CN or CHN): 31.0% Bismark completed in 0d 0h 1m 19s