Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_26psu_2_S14_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/16C_26psu_2_S14_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Ssalar/GENOMES/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 33792 Mapping efficiency: 33.8% Sequence pairs with no alignments under any condition: 52674 Sequence pairs did not map uniquely: 13534 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8605 ((converted) top strand) GA/CT/CT: 8267 (complementary to (converted) top strand) GA/CT/GA: 8295 (complementary to (converted) bottom strand) CT/GA/GA: 8623 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1489578 Total methylated C's in CpG context: 114640 Total methylated C's in CHG context: 2845 Total methylated C's in CHH context: 13439 Total methylated C's in Unknown context: 301 Total unmethylated C's in CpG context: 31821 Total unmethylated C's in CHG context: 372830 Total unmethylated C's in CHH context: 954003 Total unmethylated C's in Unknown context: 813 C methylated in CpG context: 78.3% C methylated in CHG context: 0.8% C methylated in CHH context: 1.4% C methylated in unknown context (CN or CHN): 27.0% Bismark completed in 0d 0h 1m 20s