Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/Sealice_F2_S22_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/Sealice_F2_S22_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Caligus/GENOMES/ with the specified options: -q --score-min L,0,-2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 76713 Mapping efficiency: 76.7% Sequence pairs with no alignments under any condition: 202 Sequence pairs did not map uniquely: 23085 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 19827 ((converted) top strand) GA/CT/CT: 19026 (complementary to (converted) top strand) GA/CT/GA: 18730 (complementary to (converted) bottom strand) CT/GA/GA: 19130 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2628397 Total methylated C's in CpG context: 19090 Total methylated C's in CHG context: 17622 Total methylated C's in CHH context: 309379 Total methylated C's in Unknown context: 57736 Total unmethylated C's in CpG context: 326246 Total unmethylated C's in CHG context: 330102 Total unmethylated C's in CHH context: 1625958 Total unmethylated C's in Unknown context: 131518 C methylated in CpG context: 5.5% C methylated in CHG context: 5.1% C methylated in CHH context: 16.0% C methylated in unknown context (CN or CHN): 30.5% Bismark completed in 0d 0h 2m 8s