Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/Sealice_F1_S20_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/Sealice_F1_S20_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Caligus/GENOMES/ with the specified options: -q --score-min L,0,-2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 74102 Mapping efficiency: 74.1% Sequence pairs with no alignments under any condition: 168 Sequence pairs did not map uniquely: 25730 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20180 ((converted) top strand) GA/CT/CT: 17036 (complementary to (converted) top strand) GA/CT/GA: 16881 (complementary to (converted) bottom strand) CT/GA/GA: 20005 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2833017 Total methylated C's in CpG context: 15196 Total methylated C's in CHG context: 14081 Total methylated C's in CHH context: 169990 Total methylated C's in Unknown context: 31058 Total unmethylated C's in CpG context: 385378 Total unmethylated C's in CHG context: 394150 Total unmethylated C's in CHH context: 1854222 Total unmethylated C's in Unknown context: 116816 C methylated in CpG context: 3.8% C methylated in CHG context: 3.4% C methylated in CHH context: 8.4% C methylated in unknown context (CN or CHN): 21.0% Bismark completed in 0d 0h 2m 14s