Bismark report for: /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/Sealice_F2_S22_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/TRIMG_adapt_5bp/TRIM_cat/Sealice_F2_S22_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Caligus/GENOMES/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --minins 60 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 35416 Mapping efficiency: 35.4% Sequence pairs with no alignments under any condition: 45815 Sequence pairs did not map uniquely: 18769 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8928 ((converted) top strand) GA/CT/CT: 8926 (complementary to (converted) top strand) GA/CT/GA: 8885 (complementary to (converted) bottom strand) CT/GA/GA: 8677 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1496563 Total methylated C's in CpG context: 4409 Total methylated C's in CHG context: 3794 Total methylated C's in CHH context: 28616 Total methylated C's in Unknown context: 4381 Total unmethylated C's in CpG context: 238222 Total unmethylated C's in CHG context: 243334 Total unmethylated C's in CHH context: 978188 Total unmethylated C's in Unknown context: 31773 C methylated in CpG context: 1.8% C methylated in CHG context: 1.5% C methylated in CHH context: 2.8% C methylated in unknown context (CN or CHN): 12.1% Bismark completed in 0d 0h 0m 47s