FastQCFastQC Report
Mon 22 Jul 2019
Undetermined_S0_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12609848
Sequences flagged as poor quality0
Sequence length150
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG412920.32745834842735616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA127670.10124626403109696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT1800.0116.108811
CCTGGCT1400.0113.2491
GCATGCT352.863926E-7102.9536361
CTTGGCT1300.099.785831
TGCTGCT800.099.092871
TTGGCTC5850.096.090052
GGCTGCT900.096.090051
CCAGGCT850.093.263881
CTGCGGT3600.090.084432
TGGCTCT18700.087.388713
CTGGCTC6000.086.481052
CCTGCGG1700.084.785351
GCCCTTG353.5423964E-582.362911
ATGGCTC4300.082.1234742
GTGCTCT19650.082.0647353
TGCAGAC4050.081.766223
CAGTGCT1500.081.676551
TGTGCTC9000.081.6765442
CCTGATG1200.078.073172
TCTGGCT2350.076.6675951
Produced by FastQC (version 0.11.5)