FastQCFastQC Report
Mon 22 Jul 2019
Undetermined_S0_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12609848
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCGG3000.0139.306491
CCTGGCT2700.0133.435331
GGCCTAA950.0121.355931
GCTGGCT1100.0117.908311
TCTGGCT3250.0115.2881241
GGCCTTT700.0113.229411
ACTGGCT1800.0112.085681
CTTGGCT2150.0110.596171
CTGGCTC9700.0109.196882
TTGGCTC11050.0104.3331452
TAGGCTC5450.0103.12472
TGGCTCT32900.0100.568363
CCAGGCT1600.099.075741
CTGCGGT5150.097.938952
CAGGCTC3950.096.68152
CAGCGGT3750.096.073452
CTTGCGG1350.096.073441
ATGGCTC7200.094.071912
CTGCGGA3600.094.071912
CATGCGG1000.093.67161
Produced by FastQC (version 0.11.5)