FastQCFastQC Report
Mon 22 Jul 2019
Undetermined_S0_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12417567
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG433390.3490136191735467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCT500.0129.723161
GCCTGCT1650.0113.5623551
GGCTGCT700.0113.250371
GGCCATA458.913048E-11112.106421
ACTGGCT1200.0108.102621
CTGGCTC4600.0101.835812
TGGCTCT16150.099.911333
CCTGGCT1250.098.013051
TTGGCTC5600.097.807142
TGCAGAC3550.097.398473
GCTGGCT607.2759576E-1296.091221
CTGCGGT3750.092.2475742
TGTGCTC8100.091.642552
CCTGCGG2400.090.085521
CAGGCTC3300.087.355652
CCTTGCT2250.086.48211
CCCTGCT4850.083.213011
TAGGCTC3900.083.155862
CTAGGCT1300.083.155861
GTGCTCT17800.082.960843
Produced by FastQC (version 0.11.5)