FastQCFastQC Report
Mon 22 Jul 2019
Undetermined_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12417567
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATG100.0072416267143.989751
CCTGGCT1600.0143.989751
TGCACGG100.0072416267143.989751
TCTGGCT2300.0131.46891
CCTGCGG3150.0127.990891
GCTGGCT900.0119.991451
CTTGGCT2200.0117.809791
CCAGGCT1400.0113.13481
CTGCGGA3800.0111.781522
ACTGCGG3300.0111.26481
TAGGCTC5300.0110.02992
CATGCGG800.0107.992311
GCTTGCT405.0604285E-9107.992311
GGCCTTT203.976169E-4107.992311
ACTGGCT1600.0107.992311
GCTGCGA1150.0106.427211
TTGGCTC11250.0104.952532
CTGCGGT6450.0104.922762
ATGGCTC7550.0102.9860462
TGGCTCT31500.0102.2084353