FastQCFastQC Report
Mon 5 Aug 2019
Sealice_F2_S22_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F2_S22_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81629033
Sequences flagged as poor quality0
Sequence length15-145
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCG542650.052.4114382
AGACACT75650.051.588711
GACACTG76850.050.7828182
CTTCCGA563700.049.7087973
TTCCGAT572300.048.4991574
CCGATCT574250.046.419996
TCCGATC580100.046.3685655
CGATCTG26750.044.9731987
CGATCTA278450.044.8997467
ACACTGC88650.043.8933453
CACTGCC88300.043.871764
CGATCTT135600.042.9587947
CGATCTC172700.041.996157
CTGCCAC94000.041.5176856
TGCCACA96200.040.6878857
GCCACAT96000.040.5927738
ACTGCCA98000.039.881775
GATCTAC105850.038.3380058
GATCTCC69650.038.0161368
GATCTGC8600.036.1431968
Produced by FastQC (version 0.11.5)