FastQCFastQC Report
Mon 5 Aug 2019
Sealice_F2_S22_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F2_S22_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81693579
Sequences flagged as poor quality0
Sequence length15-145
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT101850.061.181881
GTCTAGC400.003879854260.840054138-139
TCTTCCG470250.057.096162
CTTCCGA482950.054.5357743
GACACTG114500.054.121532
TTCCGAT490200.052.991094
GCCGCGT403.3744684E-452.23096126-127
TCCGATC485000.051.5106285
CCGATCT480400.051.3822066
CAGCGAG1750.049.2269949
CGATCTA243150.047.617397
ACACTGC131900.046.8943443
CACTGCC132900.046.325424
CGATCTT114500.045.8949557
GATCTAC92900.043.9545638
CTGCCAC142050.043.2605326
ACTGCCA144000.042.6747135
TGCCACA144800.042.319957
GCCACAT145150.042.138858
TCAGCGA2050.042.023048
Produced by FastQC (version 0.11.5)