FastQCFastQC Report
Mon 5 Aug 2019
Sealice_F2_S22_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F2_S22_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82103644
Sequences flagged as poor quality0
Sequence length15-145
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT74650.056.481471
GACACTG77150.054.3516542
TCTTCCG547900.051.6806372
CCGATGC1400.049.370816
CTTCCGA571100.049.0767363
TTCCGAT581800.047.8276524
ACACTGC88500.047.576213
CACTGCC89050.047.1529964
TCCGATC588250.045.6779675
CCGATCT584450.045.6595846
CGATCTG26350.045.0302287
CGATCTA283300.043.9975247
CTGCCAC96650.043.6240776
CGATCTT140450.043.06117
TGCCACA98650.042.5645457
ACTGCCA98550.042.5492365
GCCACAT99450.041.9905748
CGATCTC171250.041.4042637
GATCTGC9000.040.9596338
CGATGCT1750.039.4966937
Produced by FastQC (version 0.11.5)