FastQCFastQC Report
Mon 5 Aug 2019
Sealice_F2_S22_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F2_S22_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82172060
Sequences flagged as poor quality0
Sequence length15-145
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT99500.064.7453461
TGCCTGT500.003550807562.216576126-127
TCTTCCG473900.057.628542
GACACTG111950.057.133962
CTTCCGA486700.055.0374763
GGACCGT300.006082859454.306564130-131
TGCCGCT450.006420559753.569794138-139
TTCCGAT492700.053.4916734
CCGATCT484100.051.8279726
TCCGATC489900.051.6135675
ACACTGC131000.048.7816433
CGATCTA240700.047.626087
CGATCTT113650.047.574267
CACTGCC133300.047.4221424
ACTGCCG506.3036295E-446.015445
TAGACCG1251.2136115E-744.998627138-139
GATCTAC90600.044.3139888
CTGCCAC142850.043.808896
GATCTCC62450.043.6575668
GATCTCA73600.043.5302358
Produced by FastQC (version 0.11.5)