FastQCFastQC Report
Mon 5 Aug 2019
Sealice_F1_S20_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F1_S20_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112546995
Sequences flagged as poor quality0
Sequence length15-145
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1264960.11239393819444048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT537050.073.9778061
GACACTG660350.060.0235672
TCTTCCG3088000.057.340832
ACACTGC713950.055.4195943
CTTCCGA3215750.054.009243
CACTGCC736200.053.539414
TTCCGAT3329300.051.5858844
CTGCCAC767450.051.359356
ACTGCCA773150.051.0558555
TGCCACA775750.050.7648857
GCCACAT776650.050.6013158
CTCTTCC3572250.050.56051
CGATCTG266500.049.7439087
TCCGATC3362300.049.2732735
CCGATCT3354900.049.052846
CTTTCCG23300.048.639921
CGATCTA1406550.048.505147
CGATCTT836650.047.4517567
GATCTGC87400.046.8802228
CCGATGC9550.046.8596656
Produced by FastQC (version 0.11.5)