FastQCFastQC Report
Mon 5 Aug 2019
Sealice_F1_S20_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F1_S20_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112669067
Sequences flagged as poor quality0
Sequence length15-145
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT841150.082.596481
CTTTCCG34250.064.342981
GACACTG1118500.062.0686872
TCTTCCG2754100.059.863212
ACACTGC1220400.056.8285263
GGGCACT552.428404E-455.829212138-139
CTTCCGA2879650.055.49053
CTCTTCC3122550.054.627091
AGACGCT1700.054.5819661
CACTGCC1279200.054.030464
AGGCACT2700.053.697541
TTCCGAT3015000.052.283884
GGATCCA902.050001E-951.5496372
CAAGCTG6650.051.4522173
TCCGATG20850.051.178215
ACTGCCA1353450.050.9677625
CTGCCAC1354950.050.8942156
GGCACTG2850.050.8713532
CCGATGC5850.050.5577356
TGCCACA1364600.050.4663127
Produced by FastQC (version 0.11.5)