FastQCFastQC Report
Mon 5 Aug 2019
Sealice_F1_S20_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F1_S20_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113310068
Sequences flagged as poor quality0
Sequence length15-145
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1366350.12058504810005055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT552450.074.718071
GACACTG678100.060.614712
TCTTCCG3177900.057.6610152
ACACTGC737150.055.774733
TGCCACG2100.055.3999067
CACTGCC756950.054.1774754
CTTCCGA3324750.054.1377563
CTGCCAC788350.051.864776
ACTGCCA789950.051.8628125
TTCCGAT3443350.051.722354
CTCTTCC3647250.051.2193341
GCCACAT798700.051.0689548
TGCCACA798250.051.061267
GCTGCCA1600.050.898625
CCGATGC10750.050.86486
TCCGATC3479650.049.325645
CCGATCT3473750.049.0828826
CGATCTA1446800.048.9064647
CGATCTG277650.048.563967
CGATGCT11100.048.2128947
Produced by FastQC (version 0.11.5)