FastQCFastQC Report
Mon 5 Aug 2019
Sealice_F1_S20_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F1_S20_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113437721
Sequences flagged as poor quality0
Sequence length15-145
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT863100.082.1752241
CGGATCC1250.065.0585561
CCGATGC5150.064.2915656
CTTTCCG32300.063.6630061
GACACTG1149300.061.6966972
AGGCACT2550.061.5049361
CGATGCT5450.060.7525837
TCTTCCG2788250.060.3265152
GGCACTG2750.057.0318532
ACACTGC1247350.056.735163
TCCGATG20200.056.0750435
CTTCCGA2911250.056.0238653
CTCTTCC3173150.054.6656721
CACTGCC1310350.053.958644
TTCCGAT3044200.052.9054534
CAAGCTG6300.052.5557983
CCGATGA5750.052.531756
CGGTCTA2700.051.633777
CGATCTG214300.050.9591337
TCCGATC3034250.050.883115
Produced by FastQC (version 0.11.5)