FastQCFastQC Report
Mon 5 Aug 2019
CTRL_8C_26psu_2_S18_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_2_S18_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16932793
Sequences flagged as poor quality0
Sequence length15-145
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368630.21770182863512239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCTA100.0021684803215.47177128-129
GGACCAT150.0072932066143.64786128-129
AGGGCTC352.8346456E-4117.5784106-107
CCAGAGC1057.713854E-570.39352138-139
TGAGCCT250.004211012759.59445690-91
AGACACT26250.052.1763271
CTGCGAG700.00986937348.048378130-131
GACACTG32500.041.7383732
TCTTCCG168700.039.893162
TTCCGAT161550.037.7854354
GAGACCG1551.1195462E-437.618347132-133
ACACTGC37000.036.6620863
CGATCTG11850.036.1880267
CACTGCC37650.036.0291374
TGCCACA38250.035.2351767
CTGCCAC39050.034.625396
ACTGCCA39350.034.4726075
CTTCCGA185000.034.438713
ACGCAGC700.00303326234.383854134-135
GGGCCCC2150.002642523534.378227138-139
Produced by FastQC (version 0.11.5)