FastQCFastQC Report
Mon 5 Aug 2019
CTRL_8C_26psu_2_S18_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_2_S18_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16952981
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCAG100.0015279093242.20726132-133
GGGATCC100.008680729135.51804104-105
GGCATCC100.009880041129.77533102-103
GTGCCGG307.887591E-490.90093118-119
GGACACT200.002952565289.97172118-119
GCCGGCC402.5551017E-671.26819696-97
GCTTGAC1300.002160817670.52015138-139
AGACACT57100.065.600471
GGAGACA1501.2420479E-463.935375132-133
CAGGATC600.003340819463.18059134-135
CAGCGTG1250.003340987563.178925136-137
CGGCATC500.00507274161.117474138-139
GGTCCGG500.00429888959.28413122-123
TAGGCCG650.00458700258.320545134-135
CCGGCTA400.00478441657.703346110-111
CTGCGGC902.3582592E-456.16053134-135
CAGAGTC500.005980072554.54057118-119
CCGGTCT401.2793564E-554.3179366
GGGCACG700.006150287654.154793134-135
AAGGCTT1750.007012768652.386402138-139
Produced by FastQC (version 0.11.5)