FastQCFastQC Report
Mon 5 Aug 2019
CTRL_8C_26psu_2_S18_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_2_S18_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16940956
Sequences flagged as poor quality0
Sequence length15-145
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG398430.2351874357031563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGA300.0043826206170.31763136-137
CCAGCAG150.0056807552156.16754132-133
GTCCGGC350.008174522138.26805134-135
GCCATTG200.002870605865.646539
GGCTCAG651.5162984E-461.409283124-125
GCCTGAT450.00375123261.359634120-121
AAGCTGA1200.003968592760.492275134-135
AGACACT28300.055.2095071
ACGCTGG700.00972615248.22651130-131
GGGTCTC1005.768694E-446.85026132-133
GACACTG33950.045.7623022
TCTTCCG175250.040.4304052
ACACTGC38550.040.301573
CACTGCC38950.039.8876884
TGCCACA39150.039.4605837
ACTGCCA39700.038.9137845
CTGCCAC39700.038.9137846
TTCCGAT168450.038.5809944
GGCGCCC1100.001666809937.76308126-127
CGATCTG12500.035.711717
Produced by FastQC (version 0.11.5)