FastQCFastQC Report
Mon 5 Aug 2019
CTRL_8C_26psu_2_S18_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_2_S18_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16962624
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGAC250.0014350044247.33278138-139
CTGAGCA100.0027481238199.07378122-123
AGCCCTG100.0040438906174.96175116-117
GGGGTCC100.0059527545153.74443110-111
GGCCGAC302.811039E-4117.82596130-131
TCCGGGC652.9121095E-4116.78314134-135
TGGCCAT407.516625E-492.00932132-133
GTGGGCT1000.001305891680.06312136-137
CGACTTG400.001461730577.82263124-125
GGCCATC550.002246320769.8347134-135
GGCCATT200.002944561465.226577
AGACACT56000.064.838321
CGTGGCC650.00604963354.38121130-131
CTTGGGC600.00615986354.1336126-127
TGACAGG600.00728696251.88176124-125
TGTGCCG500.00823652550.298893114-115
GGGCCAT354.3131877E-449.6964346
GACACTG74050.049.2098772
CGCAGGA400.00947277248.55006102-103
CACGTGG700.00959025648.398582128-129
Produced by FastQC (version 0.11.5)