FastQCFastQC Report
Mon 5 Aug 2019
CTRL_8C_26psu_1_S17_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_1_S17_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13602294
Sequences flagged as poor quality0
Sequence length15-145
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG362190.26627126277376445No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA141070.10371044766419547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCAGG100.0033720478185.9165138-139
GCGACTG300.003431826862.753845134-135
GTCGGCA300.004168251559.748196130-131
AGACACT19300.055.892061
GACACTG20950.050.7095342
GGCCCCA300.00835379550.11947114-115
GCATCAG300.00835379550.11947114-115
GCCGATC5850.048.427246
ACACTGC23550.045.1110273
TCTTCCG137750.044.889352
CACTGCC23550.044.879694
TTCCGAT139900.042.3302544
TGCCACA25150.042.0248347
ACTGCCA25200.041.941145
CTGCCAC25400.041.825696
CTTCCGA146350.041.6186643
CCGATCT144300.038.0571636
GATCCAC9500.035.555799
GCCACAT29700.035.0363778
CGATCTG8450.034.8160327
Produced by FastQC (version 0.11.5)