FastQCFastQC Report
Mon 5 Aug 2019
CTRL_8C_26psu_1_S17_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_1_S17_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13616515
Sequences flagged as poor quality0
Sequence length15-145
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGCC100.0034879753183.82793138-139
CTATGGC250.001918912472.664696130-131
AGACACT33800.070.745161
AGACCGG650.002569439667.50725138-139
GCCGATC7100.059.567746
CAGTGGC300.004330960659.173183128-129
AGTCCAG300.00516926256.586525124-125
GACACTG43800.054.3457072
GGCACTG300.00612040154.2219162
CTTCCGG4050.050.8748863
TCTTCCG153850.050.4332542
GCACTGC552.2723036E-549.292653
ACACTGC48850.048.7275733
CACTGCC49750.048.064054
CGATCTG7850.047.6600277
TTCCGAT157450.045.4919974
CTTCCGA163700.045.411273
CTGCCAC52800.045.2876246
ACTGCCA52600.045.2536475
GATCCAC11100.044.45229
Produced by FastQC (version 0.11.5)