FastQCFastQC Report
Mon 5 Aug 2019
CTRL_8C_26psu_1_S17_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_1_S17_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13755386
Sequences flagged as poor quality0
Sequence length15-145
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG387130.28143884875349917No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA158270.11506038434690236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCAGC250.001692363175.00246134-135
AGGCGCT250.002238139569.89948128-129
TGGTCAC250.003741851861.398685116-117
AGACACT18250.057.9323841
GCCGATC5550.055.970976
GTGCTGC200.005670875355.2776684-85
GACACTG20000.052.3181232
CACTGCC21950.047.173544
ACACTGC22900.045.454543
ACTGCCA22900.044.9787375
TGCCACA23200.044.1622167
CTGCCAC23200.044.1622166
GATCTGC2900.043.222598
TCTTCCG142100.041.803572
GATCCAC9950.041.078939
TTCCGAT143050.039.4685554
CGATCTG8200.039.212017
GGTATGC8900.039.189612
CTTCCGA149300.038.4733663
GCCACAT26950.038.2194148
Produced by FastQC (version 0.11.5)