FastQCFastQC Report
Mon 5 Aug 2019
CTRL_8C_26psu_1_S17_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_1_S17_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13771009
Sequences flagged as poor quality0
Sequence length15-145
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT33300.073.896281
CAAGCTG800.067.901393
GCCGATC6500.066.856756
TGCCGCA250.002951939665.1853267
GCAATGC202.3675816E-456.1164442-43
GACACTG44200.055.427192
TCTTCCG160400.051.1038322
ACACTGC49250.049.6334953
CGATCTG8700.049.326067
CACTGCC50200.048.586014
GATCTGC2300.047.2357448
TTCCGAT163100.046.8606954
CTTCCGA168950.046.620663
CTGCCAC52850.045.8414656
TGCCACA52800.045.679117
ACTGCCA53300.045.4544335
GATCCAC11650.045.228739
CGCCGAT10100.044.6401185
TCGCCGA9800.043.7894634
GCCACAT56500.042.6877378
Produced by FastQC (version 0.11.5)