FastQCFastQC Report
Mon 5 Aug 2019
CTRL_16C_26psu_2_S21_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_2_S21_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13390626
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT63750.060.0616651
GGGTCTC1300.004594227358.29641138-139
CAGGAGC500.004921658457.29235130-131
CAAGCTG2600.051.542923
AGCGGCC450.00820231250.35188118-119
GACACTG83000.045.9583632
CTCTTCC535400.044.974211
TCTTCCG529350.044.7193572
ACACTGC90150.042.4194833
CACTGCC92650.041.2748684
CTTCCGA549900.041.2291153
TTCCGAT540200.040.7379074
ACTGCCA95400.039.984745
TGCCACA96250.039.681367
CTGCCAC96400.039.669266
GGCGCCC850.001539414338.37928122-123
CGATCTG61500.038.133387
GCCACAT100000.038.049728
CCGATCT534250.037.5902566
TCCGATC546250.037.517995
Produced by FastQC (version 0.11.5)