FastQCFastQC Report
Mon 5 Aug 2019
CTRL_16C_26psu_2_S21_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_2_S21_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13400582
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCC100.0042047515172.6949124-125
GTGCCAC200.00205545571.41646
AGACACT89150.066.2761151
CAAGCTG2850.061.810683
TGCTGCT751.7103614E-459.931557136-137
GGCACGG500.00533672256.132202128-129
GACACTG116650.050.6108472
AAGCTGG3650.049.5675434
GCTGGGA3600.048.9334566
ACACTGC128600.045.8708763
TCTTCCG354400.044.4001462
CACTGCC135450.043.305034
CTCTTCC390000.041.7389141
AGCTGGG4350.041.5911565
ACTGCCA143050.040.9377445
CTGCCAC143100.040.923446
TGCCACA142650.040.885667
CTTCCGA360700.040.747143
TTCCGAT353200.039.806084
GCCACAT145750.039.7875338
Produced by FastQC (version 0.11.5)