FastQCFastQC Report
Mon 5 Aug 2019
CTRL_16C_26psu_2_S21_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_2_S21_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13414512
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCCC200.0035813504182.21039128-129
GGCCAAG156.531773E-5169.97256124-125
GGCCAGA556.651803E-572.53107132-133
AGACACT63750.061.0822331
ACTGCCG250.00486034557.47445
GGAGCCG1107.726467E-752.263878130-131
TGCCGCA300.00999475247.8953367
GACACTG83250.046.4859772
CTCTTCC526250.045.1797941
TCTTCCG523750.044.6811452
ACACTGC89900.042.9406433
CAAGCTG2950.042.212843
CACTGCC92100.041.914924
TAGCCGT3754.7293724E-1141.83197138-139
CTTCCGA544000.041.25693
TTCCGAT536700.040.6311654
TGCCACA95900.039.9044577
CTGCCAC96500.039.805246
ACTGCCA96800.039.7808385
CGATCTG61200.038.8171357
Produced by FastQC (version 0.11.5)