FastQCFastQC Report
Mon 5 Aug 2019
CTRL_16C_26psu_2_S21_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_2_S21_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13425522
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCC150.002338219210.11285138-139
GGACACT408.960524E-871.37351
TGGCCAT400.002131956970.76105128-129
CAGCATG500.002526647267.79393136-137
AGACACT91700.066.677531
TCAGTCA950.003219096763.77452132-133
CAGCGCT250.00498813357.0987975
GGTCTTC502.6975188E-454.65343112-113
CGTGGCC500.006063068854.350796126-127
CAAGCTG3750.053.2922133
GACACTG121050.050.471472
CAGAGCA1200.004087790448.61248130-131
CCGGCAC805.5761763E-447.17403128-129
ACACTGC131950.046.266113
TCTTCCG361650.045.9705962
CTGTGTC757.633906E-445.963814124-125
GCTGGGA4600.044.4791376
TGCCGAA857.5173914E-444.39909128-129
CACTGCC136950.044.368484
AAGCTGG4700.043.5327724
Produced by FastQC (version 0.11.5)