FastQCFastQC Report
Mon 5 Aug 2019
CTRL_16C_26psu_1_S19_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_1_S19_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18471315
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG204240.1105714455088877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCTG459.695214E-1082.9412163
GCCAAGA600.002530511767.768005132-133
GCAGCAT150.00460533658.26242456-57
AGACACT25900.055.79021
TAGCCAG400.005503827755.696842136-137
GTGGCAC350.00632529653.772446126-127
GACACTG29350.049.231842
ACACTGC33050.043.7202533
CACTGCC33550.043.0686844
CGATCTG14450.042.4340867
TCTTCCG242150.042.4088672
TGCCACA34700.041.1803637
ACTGCCA35100.041.1667945
CTGCCAC34750.041.121116
GATCTGC4800.041.1003348
TTCCGAT235500.040.685724
GGTATGC22100.039.5673032
CTTCCGA260100.038.1906783
GCCACAT38850.036.5069438
CCGATCT237150.036.288196
Produced by FastQC (version 0.11.5)