FastQCFastQC Report
Mon 5 Aug 2019
CTRL_16C_26psu_1_S19_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_1_S19_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18487321
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCCA300.002387860368.76739132-133
AGACACT43350.062.5033871
GACACTG50350.053.708022
CGCAGCG350.00944683948.583866118-119
ACACTGC56500.047.7674523
CACTGCC56450.047.338274
TCTTCCG229800.046.8388332
ACTGCCA58300.045.562065
TGCCACA58650.045.471697
CTGCCAC59000.045.2019426
TTCCGAT223050.043.9363674
CGATCTG12350.042.671677
CTTCCGA242450.042.2869763
GCCACAT63300.042.0472568
CCGATCT214600.039.6138046
GCCGATC8100.036.1450736
GATCTGC3850.035.9487658
TCCGATC241950.035.641895
CTTGGGC850.00241786635.007324136-137
CTGCCGA802.3051901E-433.2699853
Produced by FastQC (version 0.11.5)