FastQCFastQC Report
Mon 5 Aug 2019
CTRL_16C_26psu_1_S19_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_1_S19_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18555713
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218210.11759720577700249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT28100.057.398241
GACACTG32000.050.234982
CAAGCTG651.156166E-649.297813
ACACTGC35600.045.3050583
CACTGCC35700.045.178154
TGCCACA36400.043.7224667
GTGCGCC658.461975E-443.344913134-135
CTGCCAC36800.043.247226
ACTGCCA37300.042.9538575
TCTTCCG238350.041.564112
CGATCTG14300.041.081517
GGTATGC22000.039.5689622
TTCCGAT236800.039.468094
GAGACCG1100.00161727837.995834138-139
GCCACAT41900.037.728428
CTTCCGA256700.037.6151053
GATCTGC4650.036.752598
CGCAAGC650.00196290936.526585122-123
CCGATCT235900.035.973756
ATCTGCG1057.6180004E-735.604079
Produced by FastQC (version 0.11.5)