FastQCFastQC Report
Mon 5 Aug 2019
CTRL_16C_26psu_1_S19_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_1_S19_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18573266
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAGC100.008289432137.62561132-133
GGCGCTA250.0092684515132.57948138-139
AGACACT41800.068.324111
GGGACAC250.002516563367.86295118-119
CCAGATG500.003616628761.9284138-139
GACACTG50300.056.7782942
AGTTCCG700.005637541455.359756128-129
CTGCAGT551.2747236E-554.351215122-123
GACCGGA1601.6963995E-551.788864138-139
ACACTGC55750.051.323343
GGCTTCT350.007651487451.24543122-123
CACTGCC56000.050.9039274
CAAGCTG755.525544E-849.7304423
CTGCCAC58300.048.5301366
TGCCACA58200.048.0642137
ACTGCCA59050.048.003985
TCTTCCG234700.046.65352
GCCACAT62300.044.8155568
TTCCGAT227700.043.852274
CGATCTG14250.042.252187
Produced by FastQC (version 0.11.5)