FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_4_S8_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_4_S8_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14597371
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCAC450.003335915563.204273138-139
CCCTTGG402.5452435E-455.3003582
AGACACT15250.048.954581
GACACTG16900.044.1748432
TCTTCCG169000.042.0479052
TTCCGAT170600.039.254824
CGATCTG11050.039.035687
ACACTGC19650.038.2740363
CACTGCC19650.037.992614
CTTCCGA181600.037.9124153
CTGCCAC20400.036.867036
TGCCACA20650.036.6884967
ACTGCCA21050.035.4657865
CCGATCT174400.034.911646
TCCGATC188550.032.4381875
TGGCCGT1200.003992024331.602139138-139
GCCACAT25350.029.6681428
GCCGATC4400.028.9071046
CTCTTCC254700.028.4644741
GATCTCA22900.027.0465558
Produced by FastQC (version 0.11.5)