FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_4_S8_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_4_S8_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14606752
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAGC100.0038315463178.14511104-105
GTGGTCC100.008819872134.79901136-137
CAGCATG250.001663381275.32969132-133
CCTGTTG250.002769695166.241862
AGACACT22700.062.2537351
GGTGTCC250.005299023456.233215108-109
GACACTG26650.053.0266342
TCTTCCG156050.049.594732
CGATCTG9550.048.5537457
TTCCGAT152750.046.654324
ACACTGC30800.046.2397883
CACTGCC31250.045.3972934
CTTCCGA163900.045.0297243
TGCCACA32750.043.149477
CTGCCAC32850.043.018126
GATCTGC3650.042.3459638
CCGATCT150600.042.2621086
ACTGCCA33700.042.0968935
GCCACAT37150.038.781858
TCCGATC172650.037.1203845
Produced by FastQC (version 0.11.5)