FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_4_S8_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_4_S8_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14674894
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGGA300.006934047752.537525118-119
AGACACT16200.048.8391571
GACACTG16650.047.850992
CGATCTG9650.044.7206887
CACTGCC19050.041.822094
TCTTCCG172450.041.3874972
ACACTGC19650.040.545083
TGCCACA20150.039.8138477
TTCCGAT172200.039.3587384
CTGCCAC20300.039.247116
ACTGCCA20500.038.864215
GCCGATC4050.038.2511256
CTTCCGA185750.037.3811873
CAAGCTG600.001871081736.884763
CCGATCT175050.035.2414636
CCGGTCT650.002770939834.0477035
TCCGATC190950.032.6836555
GCCACAT24850.032.283668
TCTGGTC1200.00409121131.444078138-139
CTCTTCC255650.028.1564941
Produced by FastQC (version 0.11.5)