FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_4_S8_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_4_S8_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14684836
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACAGT150.0038998667177.09528136-137
AGACACT23550.072.448361
CTGGACT200.00199605871.94638110-111
GACACTG28450.059.9704322
ACACTGC31650.053.907073
CACTGCC32350.052.7406164
AGGACAC550.00800684950.660076138-139
CTGCCAC33650.050.5389986
ACTGCCA33950.050.0924075
TGCCACA33950.050.0924077
TCTTCCG160250.046.2397462
GCCACAT37850.044.930978
CAAGCTG507.7096105E-444.1723983
TTCCGAT156600.042.9453854
CTTCCGA170500.041.87313
ATCTGGC550.001232327540.1567239
CCGATCT154950.038.8058556
CGATCTG9750.037.376647
GATCTGC2700.036.810338
TCCGATC172250.035.4533165
Produced by FastQC (version 0.11.5)