FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_3_S7_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_3_S7_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14103212
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG175200.12422702005755852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCAT100.0044236355169.78995134-135
GGGCCAC250.00159371676.14616116-117
GGCCACA356.656976E-572.52016116-117
CCGGCCC250.006123532554.2149494-95
TGGCACG400.00904105849.12662118-119
AGACACT17050.046.923871
GACACTG18650.043.6974222
CACTGCC20550.038.206264
ACACTGC21050.038.006953
GGGGCCA650.001653255137.8261114-115
TGCCACA21350.037.473037
GGGAGCC2109.173122E-636.475574138-139
ACTGCCA21950.036.4485785
CTGCCAC22000.036.365876
AGCGCCG600.002049055236.2083106-107
GGTATGC13200.034.6341632
TCTTCCG124600.034.1785472
CCACGGT2050.003318494232.817085134-135
TTCCGAT122250.032.4371954
GCCACAT25750.031.4558328
Produced by FastQC (version 0.11.5)