FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_3_S7_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_3_S7_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14117159
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC200.002515278205.04971138-139
CACGTGG400.002429159868.470314136-137
AGACACT27350.058.470011
CTTAGGC750.0077420551.09267138-139
GACACTG32550.048.9770772
CAAGCTG553.8889975E-545.007943
ACACTGC35750.044.592483
TCTTCCG128500.044.5777552
CACTGCC36850.043.127014
CTGCCAC37700.042.6799436
TGCCACA38050.042.417477
ACTGCCA38200.041.7324945
CGATCTG6700.041.3804327
TTCCGAT125650.040.9781764
CTTCCGA133550.040.518943
GCCACAT41650.038.7512668
CCGATCT125100.036.0135466
GTCCAGT900.002130841135.92057130-131
CCGGTCT701.6106354E-435.363386
GTCTGCC851.8868553E-434.423687110-111
Produced by FastQC (version 0.11.5)