FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_3_S7_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_3_S7_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14147842
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189330.13382252925923263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGCA250.00434457159.126358100-101
AGACACT15500.048.1759641
GACACTG18000.041.7609832
CGATCTG7950.041.3280227
ACACTGC19700.038.1571123
CACTGCC19850.037.617984
TCTTCCG126850.037.1642882
GGTATGC13200.036.9588472
ACTGCCA20750.036.2265245
TGCCACA20800.036.1394427
CTGCCAC20900.035.9665266
TTCCGAT125650.035.3796654
CTTCCGA133850.034.3279653
GATCTGC2350.033.8937768
ATCTGCC1302.0162042E-730.634769
CCGATCT131350.030.1682996
GCCACAT25550.029.031088
TCGGGAC3150.028.4465625
CGGATCA3802.5465852E-1128.350687136-137
TCCGATC141750.028.3412045
Produced by FastQC (version 0.11.5)