FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_3_S7_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_3_S7_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14163220
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAG250.0050605754162.32149138-139
CGGGTCC100.0069790217145.7796124-125
CTGAGGC300.008728539135.26791138-139
AGGCCAA300.001672858375.22175126-127
GCACCTG200.001751591374.355122
CTGCTGA350.003082763964.475784126-127
AGACACT25800.061.2901341
TCAGCTG400.004046009460.198833130-131
ACAGGGC701.364486E-553.72982126-127
CCGGTCT404.3676712E-449.570086
CAAGCTG502.1981317E-549.5700763
GACACTG32700.048.054172
TCTTCCG133400.045.4454272
CGATCTG6900.044.5412257
ACACTGC36500.043.1870773
TTCCGAT130450.042.483214
CACTGCC36950.042.392814
CAGATGG1957.894414E-1041.824436136-137
CTTCCGA140650.041.3759463
CTGCCAC38550.040.7619066
Produced by FastQC (version 0.11.5)