FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_2_S6_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_2_S6_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12854445
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144860.11269253553926287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCAGC100.0068463963146.7181134-135
AGACACT16800.048.248031
GACACTG16650.048.054162
ATCTGCC1500.045.3218469
GATCTGC2700.042.610288
ACCAGAG1103.360432E-642.22035136-137
ACACTGC19050.042.0000883
CACTGCC19050.041.725584
CTGCCAC19550.040.926086
TGCCACA19600.040.5548677
ACTGCCA19600.040.5547075
TCTTCCG138150.038.9888232
CGATCTG8850.038.408347
TTCCGAT137800.036.9247364
CTTCCGA148550.035.555153
GCCACAT23850.033.1088458
CCGATCT142500.032.5142946
GTGAGCA750.004255854531.19168118-119
GTCTGGC950.004464482430.88802134-135
TCCGATC154250.030.1730065
Produced by FastQC (version 0.11.5)