FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_2_S6_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_2_S6_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12866543
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCTC100.007884165139.95262130-131
GGCGCCT100.008599736135.94365128-129
AGCCAGT200.008788065134.96126128-129
AGACCGG408.994652E-6108.55202138-139
ACGGCAT800.002964037265.1172138-139
AGACACT28150.062.79741
GACACTG33050.053.1714862
ACACTGC35900.048.950163
TCTTCCG149150.048.1427652
CACTGCC36650.047.663894
CGATCTG8350.046.837477
TGCCACA38100.045.7130477
ACTGCCA38500.045.5089875
CTGCCAC38900.045.1750836
TTCCGAT143850.044.913834
CTTCCGA155850.043.864643
CCTGTTG500.001021665641.7167362
GCCACAT42250.041.5931558
CCGATCT146500.039.0476
GCCGATC4450.038.669866
Produced by FastQC (version 0.11.5)