FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_2_S6_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_2_S6_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12923530
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155930.12065588890960906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCCA100.0097226845130.47433126-127
AGACACT16100.050.7454151
GACACTG17450.046.819372
ATCTGCC1900.044.1024559
ACACTGC19600.041.950773
GATCTGC2900.041.5361488
CACTGCC19900.041.3183484
TGCCACA20250.040.6042027
TCCGGTC657.478513E-540.2858965
CTGCCAC20600.039.6600956
CGATCTG10250.039.3426177
ACTGCCA21100.038.9684945
TCTTCCG137200.038.5916562
GGTATGC12400.037.589342
TTCCGAT136650.036.6007654
CTTCCGA146850.034.9857673
GCCACAT24450.033.200858
CCGATCT142250.032.2881936
TCCGATC150850.030.8293275
CTTCCGG4900.027.7890473
Produced by FastQC (version 0.11.5)